منابع مشابه
Sexing of carcass remains of the Sika deer (Cervus nippon) using PCR amplification of the Sry gene.
In order to determine the sex of carcass remains of the Sika deer (Cervus nippon), we improved a polymerase chain reaction (PCR) technique for amplification of the Sika deer Sry, a male-specific DNA region on the mammalian Y chromosome. From the nucleotide sequence of the Sry region obtained here, PCR primers, MT1 and MT2, capable of amplifying a shorter Sry region were newly designed, and a mi...
متن کاملPCR effectiveness for sexing Schistosoma mansoni cercariae: application for sexing clonal cercarial populations.
Schistosoma mansoni is a digenean parasite known for its importance as a human pathogen. Compared with the other genera belonging to the digenean families, the Schistosoma genus is characterized by having acquired gonochorism from hermaphroditic ancestors [1]. Sex is genetically determined in the egg [2] and female schistosome is heterogametic (ZW) such as lepidoptera, reptiles and birds. A mar...
متن کاملSexing single bovine blastomeres using TSPY gene amplification.
The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each embryo. A total of 36 single blastomeres and the remaining cells o...
متن کاملMorphological method for sexing anopheline larvae.
BACKGROUND & OBJECTIVES Most of autocidal control of malaria vectors relies on the rearing and release of large numbers of sterile male into a wild population and it would be crucial to separate the males from females before release. This could result in enormous economic benefits in the mass rearing and raise the efficiency of the field operations. The development of genetic sexing of mosquito...
متن کاملBovine Papillomavirus in Brazil: Detection of Coinfection of Unusual Types by a PCR-RFLP Method
Bovine papillomavirus (BPV) is recognized as a causal agent of benign and malignant tumors in cattle. Thirteen types of BPV are currently characterized and classified into three distinct genera, associated with different pathological outcomes. The described BPV types as well as other putative ones have been demonstrated by molecular biology methods, mainly by the employment of degenerated PCR p...
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ژورنال
عنوان ژورنال: Brazilian Journal of Veterinary Research and Animal Science
سال: 2002
ISSN: 1413-9596
DOI: 10.1590/s1413-95962002000300007